Biotinylated Human LAG-3, Fc,Avitag (LA3-H82Fb) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
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This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 75.0 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LAG-3, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) is more than 90% and the molecular weight of this protein is around 200-260 kDa verified by SEC-MALS.
Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) with a linear range of 0.1-3 ng/mL (QC tested).
Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, His Tag (Cat. No. FG1-H52Hy): Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) binding reactions. The half maximal inhibitory concentration (IC50) is 0.16695 μg/mL (Routinely tested).
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.