Recombinant Proteins iso9001 ISO13485
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Biotinylated Human B7-H3 (4Ig) / B7-H3b Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309/PRO352,B7 homolog 3

表达区间及表达系统(Source)

Biotinylated Human B7-H3 (4Ig), His,Avitag (B7B-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).

Predicted N-terminus: Gly 27

蛋白结构(Molecular Characterization)

Online(Gly 27 - Thr 461) Q5ZPR3-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 50.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human B7-H3 (4Ig), His,Avitag (Cat. No. B7B-H82E8) SDS-PAGE gel

Biotinylated Human B7-H3 (4Ig), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human B7-H3 (4Ig), His,Avitag (Cat. No. B7B-H82E8) MALS images

The purity of Biotinylated Human B7-H3 (4Ig), His,Avitag(Cat. No. B7B-H82E8) was more than 90% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3 (4Ig), His,Avitag (Cat. No. B7B-H82E8) with a linear range of 0.3-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Biotinylated Human B7-H3 (4Ig), His,Avitag (Cat. No. B7B-H82E8) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Biotinylated Human B7-H3 (4Ig), His,Avitag (Cat. No. B7B-H82E8) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.

 

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