膜杰作
Star Staining
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
Star Ribbon
分子别名（Synonym）
PDL1,PD-L1,CD274,B7-H1,B7H1,B7-H,B7H,PDCD1L1,PDCD1LG1,ADC target
表达区间及表达系统（Source）
Cynomolgus / Rhesus macaque PD-L1, His Tag (PD1-C52H4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus.
Predicted N-terminus: Phe 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.1 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus / Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) is more than 90% and the molecular weight of this protein is around 27-40 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Report
活性（Bioactivity）-SPR
Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Cynomolgus PD-1, Fc Tag (Cat. No.PD1-C5254) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 1.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
文献引用（Citations）
Authors: Geuijen C, Tacken P, Wang LC, et al.
Journal: Nature communications 2021
Application: SPR
Authors: I Altintas, D Satijn, BE Van Den, D Verzijl
Journal: US20200239579A1 2020
Application: BLI
Authors: X Qian, et al
Journal: US20190330348A1 2019
Application: ELISA
背景（Background）
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
