Recombinant Proteins iso9001 ISO13485
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Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)

分子别名(Synonym)

FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32

表达区间及表达系统(Source)

Mouse CD32b, His Tag (SPR & BLI verified) (CDB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657.1).

Predicted N-terminus: Thr 40

蛋白结构(Molecular Characterization)

Online(Thr 40 - Arg 217) NP_001070657.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) SDS-PAGE gel

Mouse CD32b, His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

活性(Bioactivity)-SPR

Biotinylated Human SPR

Immobilized Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) on NTA-Series S sensor chip, can bind OKT3 with an affinity constant of 0.86 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Immobilized OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 0.55 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) on NTA Biosensor, can bind OKT3 with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) on NTA Biosensor, can bind Herceptin with an affinity constant of 2.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded OKT3 on Protein G Biosensor, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Herceptin on Protein G Biosensor, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

 

前沿进展

 
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Fc gamma RIIB / CD32b靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor II-b
中文全称:低亲和力免疫球蛋白γFc区受体II-B
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
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