Recombinant Proteins iso9001 ISO13485
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Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His,Avitag™&Tag Free

分子别名(Synonym)

Integrin alpha V beta 8,ITGAV&ITGB8

表达区间及表达系统(Source)

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (IT8-H82W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).

Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)

蛋白结构(Molecular Characterization)

Online(Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8)) NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.7 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 145-170 kDa (ITGAV) and 88-95 kDa (ITGB8) respectively due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT8-H82W5) SDS-PAGE gel

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TGFB1, His Tag (Cat. No. TG1-H524x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT8-H82W5) with a linear range of 5-156 ng/mL (QC tested).

Protocol

背景(Background)

Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

 

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