Cynomolgus PD-1, His Tag (PD1-C5223) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ3).
Predicted N-terminus: Leu 25
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus PD-1, His Tag (Cat. No. PD1-C5223) at 2 μg/mL (100 μL/well) can bind Sintilimab with a linear range of 0.4-6 ng/mL (QC tested).
Immobilized Cynomolgus PD-1, His Tag (Cat. No. PD1-C5223) at 1 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) with a linear range of 0.156-2.5 μg/mL (Routinely tested).
Opdivo(Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Cynomolgus PD-1, His Tag (Cat. No. PD1-C5223) with an affinity constant of 18.1 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Opdivo (Nivolumab) on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag (Cat. No. PD1-C5223) with an affinity constant of 17.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag (Cat. No. PD1-C5223) with an affinity constant of 3.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.