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Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)

分子别名(Synonym)

CEACAM-5,CD66e,CEA,Meconium antigen 100

表达区间及表达系统(Source)

Human CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).

Predicted N-terminus: Lys 35

蛋白结构(Molecular Characterization)

Online(Lys 35 - Ala 685) P06731

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CEACAM-5, His Tag (Cat. No. CE5-H5226) SDS-PAGE gel

Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CEACAM-5, His Tag (Cat. No. CE5-H5226) MALS images

The purity of Human CEACAM-5, His Tag (Cat. No. CE5-H5226) was more than 95% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CEACAM-5, His Tag (Cat. No. CE5-H5226) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 Antibody at 1 μg/mL (100 μL/well) can bind Human CEACAM-5, His Tag (Cat. No. CE5-H5226) with a linear range of 10-39 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CEACAM-5, His Tag (Cat. No. CE5-H5226) with an affinity constant of 8.41 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

 

前沿进展

 
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CEACAM-5靶点信息
英文全称:Carcinoembryonic antigen related cell adhesion molecule 5
中文全称:癌胚抗原相关细胞粘附分子5
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:12详情
最高研发阶段:临床三期
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