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 >  Protein>Glypican 3 >GP3-HF258

FITC-Labeled Human Glypican 3 / GPC3 Protein, Fc Tag

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). It is the FITC labeled form of Human Glypican 3, Fc Tag (Cat. No. GP3-H5258).

Predicted N-terminus: Gln 25 & Ser 359

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 25 - His 559) P51654-1

This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 49.3 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 87-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 3-5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) SDS-PAGE gel

FITC-Labeled Human Glypican 3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human GPC3 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) with a linear range of 0.078-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of Anti-Glypican 3 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

 

前沿进展

 
 
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Glypican 3靶点信息
英文全称:Glypican-3
中文全称:磷脂酰肌醇聚糖3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:31详情
最高研发阶段:临床二期
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