B7-H6,NCR3LG1,B7 Homolog 6
Cynomolgus B7-H6 (B76-C52Ha) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Asp 259 (Accession # XP_005578557.1).
Predicted N-terminus: Asp 25
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.4 kDa. The protein migrates as 38-50 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus B7-H6 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human NKp30, Fc Tag (Cat. No. NC3-H5259) at 5 μg/mL (100 μL/well) can bind Cynomolgus B7-H6 (Cat. No. B76-C52Ha) with a linear range of 0.625-5 μg/mL (QC tested).
The B7 family of genes is essential in the regulation of the adaptive immune system. one of which is the recently discovered B7H6. Humans and rats have a single B7H6 gene; however, many B7H6 genes were detected in a single large cluster in the Xenopus genome. Chimeric antigen receptor (CAR) T-cell therapies have demonstrated durable and potentially curative therapeutic efficacy against B-cell leukemia in clinical trials. In this study, B7H6, a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.