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 >  Protein>SIRP beta >SIA-H5257

Human SIRP beta / CD172b Protein, Fc Tag (MALS verified)

分子别名(Synonym)

CD172b antigen,CD172b,DKFZp686A05192,FLJ26614,SIRP beta 1,SIRPB1,SIRP-BETA-1,9930027N05Rik,SIRP-beta

表达区间及表达系统(Source)

Human SIRP beta, Fc Tag (SIA-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 30 - Leu 371 (Accession # O00241-1).

Predicted N-terminus: Glu 30

Request for sequence

蛋白结构(Molecular Characterization)

Online(Glu 30 - Leu 371) O00241-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 63.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human SIRP beta, Fc Tag (Cat. No. SIA-H5257) SDS-PAGE gel

Human SIRP beta, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human SIRP beta, Fc Tag (Cat. No. SIA-H5257) MALS images

The purity of Human SIRP beta, Fc Tag (Cat. No. SIA-H5257) is more than 90% and the molecular weight of this protein is around 130-158 kDa verified by SEC-MALS.

Report

背景(Background)

Signal-regulatory protein beta-1 (SIRP beta 1) also known as SIRPβ1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily. SIRPβ1 is a transmembrane protein that has three Ig-like domains in its extracellular region and a short cytoplasmic tail. Human SIRPβ1 is expressed on monocytes and granulocytes but not on lymphocytes. SIRP beta 1 contains short cytoplasmic domains that lack cytoplasmic sequence motifs capable of recruiting SHP-2 and SHP-1. In addition, they contain a single basic lysine residue within the hydrophobic transmembrane domain.

 

 

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