搜索
 >  其他蛋白>IL-3 R alpha >ILA-H52H6

Human IL-3 R alpha / CD123 Protein, His Tag (MALS & SPR verified)

分子别名(Synonym)

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统(Source)

Human IL-3 R alpha, His Tag (ILA-H52H6) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).

Predicted N-terminus: Thr 19

蛋白结构(Molecular Characterization)

Online(Thr 19 - Arg 305 ) P26951-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-3 R alpha, His Tag (Cat. No. ILA-H52H6) SDS-PAGE gel

Human IL-3 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL-3 R alpha, His Tag (Cat. No. ILA-H52H6) MALS images

The purity of Human IL-3 R alpha, His Tag (Cat. No. ILA-H52H6) was more than 90% and the molecular weight of this protein is around 47-57 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-3 R alpha, His Tag (Cat. No. ILA-H52H6) captured on CM5 Chip via anti-His antibody can bind Human IL-3, Tag Free with an affinity constant of 0.253 μM as determined in SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

 

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
IL-3 R alpha靶点信息
英文全称:Interleukin-3 receptor subunit alpha
中文全称:白细胞介素-3受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:19详情
最高研发阶段:批准上市
查看更多信息

消息提示

请输入您的联系方式,再点击提交!

确定