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ELISA Assay Kit for Anti-HER-2 h-mAb in Human Serum

For research use only.

Background/背景

How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development. HER-2 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Herceptin®. Many investigational anti-HER-2 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.

Application/应用

It is for research use only.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store all items at -20℃. After reconstitution, the stock solution should be kept at -80℃.

It is recommended not to freeze thaw more than 3 times.

This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ for 1 year in lyophilized state; -80℃ for 4 months under sterile conditions after reconstitution.

Assay Principles/原理

The enclosed ELISA assay kit for anti-HER-2 h-mAb in mouse serum is based on an enzyme immunoassay (ELISA) between recombinant HER-2 protein (ECD) and a biotinylated anti-HER-2 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-HER-2 antibodies in mouse serum.

The assay only involves the following steps:

1. Coat the plate with human HER-2;

2. Add the mixture of your sample and biotinylated anti-HER-2 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

4. Record the OD readings and analyze the serum concentration.

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Her2靶点信息
英文全称:Receptor protein-tyrosine kinase erbB-2
中文全称:受体蛋白酪氨酸激酶 erbB-2
种类:Homo sapiens
上市药物数量:24详情
临床药物数量:126详情
最高研发阶段:批准上市
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