Biotinylated Human NTB-A, His,Avitag (NTA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Met 226 (Accession # Q96DU3-1).
Predicted N-terminus: Gln 22
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 26.8 kDa. The protein migrates as 35-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human NTB-A, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The SLAM family of receptors is composed of six members named SLAM (CD150); 2B4 (CD244); Ly-9 (CD229); CD84; natural killer, T and B cell antigen (NTB-A; Ly108 in the mouse. Most, if not all,immune cell types express more than one SLAM family receptor. All six SLAM-related genes are located within a 400–500 kilobase (kb) genomic segment on chromosome 1 in humans and mice. This feature implies that the genes that encode the SLAM family were created by serial gene duplication.