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Biotinylated Human Her2 / ErbB2 Protein, His,Avitag™, premium grade

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1

表达区间及表达系统(Source)

Biotinylated Human Her2, His,Avitag, premium grade (HE2-H82E2) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).

Predicted N-terminus: Thr 23

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

蛋白结构(Molecular Characterization)

Her2 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 72.9 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5 ng/μg of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02 ng/μg of protein tested by qPCR.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Her2 SDS-PAGE

Biotinylated Human Her2, His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Her2 SEC-MALS

The purity of Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) is more than 90% and the molecular weight of this protein is around 70-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-FACS

Her2 FACS

2e5 of Anti-Her2 CAR-293 were stained with 100 μL of 1 μg/mL of Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol

 

活性(Bioactivity)-ELISA

Her2 ELISA

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

Her2 ELISA

Immobilized Trastuzumab Biosimilar at 1 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

Her2 ELISA

Immobilized Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Trastuzumab Biosimilar with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

Her2 SPR

Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) immobilized on SA Chip can bind Trastuzumab with an affinity constant of 7.82 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

文献引用(Citations)

 

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Her2靶点信息
英文全称:Receptor protein-tyrosine kinase erbB-2
中文全称:受体蛋白酪氨酸激酶 erbB-2
种类:Homo sapiens
上市药物数量:35详情
临床药物数量:200详情
最高研发阶段:批准上市
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