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Human CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD27,TNFRSF7,S152,T14,Tp55

表达区间及表达系统(Source)

Human CD27, Mouse IgG2a Fc Tag, low endotoxin (CD7-H5257) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # P26842-1).

Predicted N-terminus: Thr 21

蛋白结构(Molecular Characterization)

Online(Thr 21 - Ile 192) P26842-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 46.2 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) SDS-PAGE gel

Human CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Fc Tag (Cat. No. TN7-H526x) with a linear range of 0.2-4 ng/mL (QC tested).

Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Flow Cytometry assay shows that Human CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) can bind to Raji cells Surface Human CD70. The concentration of CD27 used is 10 μg/mL (Routinely tested).

Protocol

 

背景(Background)

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

 

前沿进展

 
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CD27靶点信息
英文全称:CD27 antigen
中文全称:CD27抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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