分子别名（Synonym）
PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
表达区间及表达系统（Source）
Biotinylated Mouse CD155, Fc,Avitag (CD5-M82F7) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Leu 348 (Accession # Q8K094-1).
Predicted N-terminus: Asp 29
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 63.5 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Biotinylation）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Mouse CD155, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Mouse TIGIT, Fc Tag (Cat. No. TIT-M5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD155, Fc,Avitag (Cat. No. CD5-M82F7) with a linear range of 20-313 ng/mL (QC tested).
Protocol
背景（Background）
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.
