搜索
 >  其他蛋白>Integrin alpha 5 beta 1 >IT1-H52W5

Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His Tag&Tag Free

分子别名(Synonym)

Integrin alpha 5 beta 1,ITGA5&ITGB1

表达区间及表达系统(Source)

Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).

Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1)

蛋白结构(Molecular Characterization)

Online(Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)) P08648-1 (ITGA5) & P05556-1 (ITGB1)

Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 110.7 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGA5) and 100-120 kDa (ITGB1) respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W5) SDS-PAGE gel

Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W5) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

 

文献引用(Citations)

背景(Background)

Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.

 

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
Integrin alpha 5 beta 1靶点信息
英文全称:Integrin alpha-5/beta-1
中文全称:整合素α-5/β-1复合体
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:3详情
最高研发阶段:批准上市
查看更多信息

消息提示

请输入您的联系方式,再点击提交!

确定