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Biotinylated Human PD-L1 / B7-H1 Protein, Fc,Avitag™ (MALS verified)

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

Biotinylated Human PD-L1, Fc,Avitag (PDL-H82F2) is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Online(Phe 19- Arg 238) Q9NZQ7-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) SDS-PAGE gel

Biotinylated Human PD-L1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) MALS images

The purity of Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) was more than 90% and the molecular weight of this protein is around 120-135 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) with a linear range of 10-313 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-Human PD-L1 MAb with a linear range of 0.1-3 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-AlphaLISA

Biotinylated Human AlphaLISA

Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) at 1 μg/mL (5 μL/well) can bind Human PD-1 (Cat. No. PD1-H5221) with a linear range of 0.04-1.25 μg/ml (Routinely tested).

Protocol

Biotinylated Human AlphaLISA

AlphaLISA shows that the binding of Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) to Human PD-1 (Cat. No. PD1-H5221) was inhibited by increasing concentration of Opdivo. The IC50 is 0.287 μg/mL (Routinely tested).

Protocol

 

背景(Background)

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

 

前沿进展

 
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PD-L1靶点信息
英文全称:Programmed death-ligand 1
中文全称:程序性死亡配体1
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:59详情
最高研发阶段:临床三期
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