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Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein, His Tag&Tag Free

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分子别名(Synonym)

Integrin alpha E beta 7,ITGAE&ITGB7

表达区间及表达系统(Source)

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).

Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)

Request for sequence

蛋白结构(Molecular Characterization)

Integrin alpha E beta 7 Structure

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). The protein migrates as 100-115 kDa and 135-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Integrin alpha E beta 7 SDS-PAGE

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-SPR

Integrin alpha E beta 7 SPR

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W7) immobilized on CM5 Chip can bind Human E-Cadherin, Fc Tag, premium grade (Cat. No. ECD-H5250) with an affinity constant of 0.148 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Integrin alpha E beta 7 consist of two major subunits: Integrin alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) and Integrin beta-7 is an integrin protein that in humans is encoded by the ITGB7 gene. Integrin alpha E beta 7 (CD103) is expressed mainly by cells of the T lymphocyte lineage within mucosal tissues. This is a strikingly narrow pattern of expression compared with that of other integrins. Lymphocytes expressing alpha E beta 7 are abundant in the gut and comprise a major part of the total T cell complement of the body.
The effectiveness of lung transplantation is marred by the relatively high incidence of rejection. The lung normally contains a large population of lymphocytes in contact with the airway epithelium, a proportion of which expresses the mucosal integrin, alpha(E)(CD103)beta(7). This integrin is not a homing receptor, but is thought to retain lymphocytes at the epithelial surface.

 

前沿进展

 
 
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Integrin alpha E beta 7靶点信息
英文全称:Integrin alpha-E/beta-7
中文全称:整合素αEβ7
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床三期
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