Recombinant Proteins iso9001 ISO13485
搜索
 >  生物标记素蛋白>IL-17 RA >ILR-H82E5

Biotinylated Human IL-17 RA / CD217 Protein, His,Avitag™

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Biotinylated Human IL-17 RA, His,Avitag (ILR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).

Predicted N-terminus: Leu 33

蛋白结构(Molecular Characterization)

Online(Leu 33 - Trp 320) Q96F46-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 37.2 kDa. The protein migrates as 55-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-17 RA, His,Avitag (Cat. No. ILR-H82E5) SDS-PAGE gel

Biotinylated Human IL-17 RA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, His,Avitag (Cat. No. ILR-H82E5) with a linear range of 0.2-8 ng/mL (QC tested).

Protocol

背景(Background)

Interleukin-7 receptor subunit alpha (IL-7 RA or IL7Ra) is also known as cluster of differentiation 127 (CD127). IL-7 RA is a type I cytokine receptor and is a subunit of the functional Interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. IL-7 RA has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V(D)J recombination[citation needed]. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation.

 

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
IL-17 RA靶点信息
英文全称:Interleukin-17 receptor A
中文全称:白细胞介素-17受体A亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
查看更多信息
推荐会议
查看全部会议

消息提示

请输入您的联系方式,再点击提交!

确定