膜杰作
Star Staining
分子别名（Synonym）
CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
表达区间及表达系统（Source）
Biotinylated Human 2B4 Protein, Avitag,His Tag (2B4-H82E9) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).
Predicted N-terminus: Cys 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag
The protein has a calculated MW of 25.4 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human 2B4 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human 2B4 Protein, Avitag,His Tag (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human 2B4 Protein, Avitag,His Tag (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48 Protein, Mouse IgG2a Fc Tag (Cat. No. BC1-H5258) with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
背景（Background）
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
