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Human TNFSF11 / RANKL / CD254 Protein, His Tag (active trimer) (MALS verified)

分子别名(Synonym)

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统(Source)

Human TNFSF11, His Tag (active trimer) (RAL-H5240) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Online(Gly 64 - Asp 245) AAC51762.1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.4 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) SDS-PAGE gel

Human TNFSF11, His Tag (active trimer) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) MALS images

The purity of Human TNFSF11, His Tag (active trimer) (MALS verified) (Cat. No. RAL-H5240) was more than 85% in HP-SEC, and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human OPG, Fc Tag (Cat. No. TNB-H5259) at 2 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-TNFSF11 (human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.274 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Anti-TNFSF11 MAb (human IgG1) on AHC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.239 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

 

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TNFSF11靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 11
中文全称:肿瘤坏死因子配体超家族成员11
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:18详情
最高研发阶段:批准上市
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