Recombinant Proteins iso9001 ISO13485
搜索
 >  生物标记素蛋白>EMMPRIN >CD7-H82E0

Biotinylated Human EMMPRIN / CD147 Protein, Avitag™,His Tag (MALS verified)

分子别名(Synonym)

Basigin,BSG,5F7,CD147,EMMPRIN,M6,OK,TCSF

表达区间及表达系统(Source)

Biotinylated Human EMMPRIN, Avitag,His Tag (CD7-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - His 205 (Accession # P35613-2).

Predicted N-terminus: Ala 22

蛋白结构(Molecular Characterization)

Online(Ala 22 - His 205) P35613-2

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 22.8 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) SDS-PAGE gel

Biotinylated Human EMMPRIN, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

SEC-MALS

Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) MALS images

The purity of Biotinylated Human EMMPRIN, Avitag,His Tag(Cat. No. CD7-H82E0) was more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-EMMPRIN Antibody, Mouse IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-EMMPRIN Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) with a linear range of 0.2-5 ng/mL (Routinely tested).

Protocol

背景(Background)

CD147 is also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.

 

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
EMMPRIN靶点信息
英文全称:Basigin
中文全称:Basigin蛋白
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:3详情
最高研发阶段:批准上市
查看更多信息
推荐会议
查看全部会议

消息提示

请输入您的联系方式,再点击提交!

确定