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 >  Protein>Nectin-2 >CD2-H82F8

Biotinylated Human Nectin-2 / CD112 Protein, Fc,Avitag™ (HPLC verified)

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分子别名(Synonym)

CD112,Nectin-2,PVRL2

表达区间及表达系统(Source)

Biotinylated Human Nectin-2, Fc,Avitag (CD2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).

Predicted N-terminus: Gln 32

Request for sequence

蛋白结构(Molecular Characterization)

Nectin-2 Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 64.0 kDa. The protein migrates as 66 kDa under reducing (R) condition, and 130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Nectin-2 SDS-PAGE

Biotinylated Human Nectin-2, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Nectin-2 SEC-HPLC

The purity of Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Nectin-2 ELISA

Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PVG-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-13 ng/mL (QC tested).

Protocol

Nectin-2 ELISA

Immobilized Human PVRIG, Fc Tag (Cat. No. PVG-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-16 ng/mL (Routinely tested).

Protocol

Nectin-2 ELISA

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Protocol

 

背景(Background)

Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

 

前沿进展

 
 
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Nectin-2靶点信息
英文全称:Transmembrane protein PVRIG
中文全称:跨膜蛋白PVRIG
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:临床二期
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