膜杰作
Star Staining
ViruStop
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
表达区间及表达系统（Source）
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (CDE-M120a) is recombinantly produced from human 293 cells (HEK293).
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (CDE-M120a), designed for preclinical stage, has the same activity and performance with GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (GMP-MC0323), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
亚型（Isotype）
Mouse IgG2a/kappa
特异性（Specificity）
The cross-reactivity with other species has not been tested yet.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
蛋白A残留(Protein A)
<5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/μg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by Membrane Filtration Method described in CP<1101>.
支原体（Mycoplasma）
Negative
运输（Shipping）
This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
For long term storage, the product should be stored at liquid state at -70°C.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4°C for 12 months under sterile condition;
- -70°C for 24 months.
电泳（SDS-PAGE）
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）-Cell based assay
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone OKT3), premium grade (CDE-M120a) stimulates secretion of IL-2 by PBMC stimulated with 10 ng/mL Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The EC50 for this effect is 0.12 ng/mL (QC tested).
Protocol
背景（Background）
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
