CD24 & Siglec-10蛋白 - 北京百普赛斯生物科技股份有限公司

背景

癌细胞表面表达特定的“别吃我”的信号分子（如CD24、CD47、PD-L1、B2M）后会被免疫细胞识别为正常细胞，从而逃避肿瘤微环境中巨噬细胞的吞噬清除。最早发现的“别吃我”信号蛋白是PD-1和CTLA-4，2019年7月发表在Nature的一项研究表明CD24是卵巢癌和三阴乳腺癌细胞表达的另一个“别吃我”信号蛋白，被癌细胞用来保护自己，CD24是开发癌症免疫疗法潜在靶点。

CD24（Cluster of Differentiation 24）是一种高度糖基化的糖基磷脂酰肌醇锚定的细胞表面蛋白。卵巢癌和三阴乳腺癌细胞表面上表达的CD24蛋白可以与免疫细胞上的Siglec-10蛋白相互作用来传递免疫抑制性信号，从而抑制破坏性炎症反应。

目前，市面的CD24蛋白大多都只有与抗体结合的数据，缺少与Siglec-10蛋白结合活性的验证，而CD24抗体药研发的关键点在于其能够有效地阻断CD24与Siglec-10的结合。为了解决这些问题，ACROBiosystems开发了不同标签的CD24蛋白以及CD24过表达细胞株，并验证了细胞株与Siglec-10的结合活性。另外还验证了抗CD24中和抗体SN3可以有效的阻断Siglec-10与CD24过表达细胞株的结合。

产品列表

Molecule	Cat. No.	Species	Product Description
CD24	CD4-H82E9	Human	Biotinylated Human CD24 Protein, His,Avitag™
	CD4-H82F5	Human	Biotinylated Human CD24 Protein, Fc,Avitag™
	CD4-H5254	Human	Human CD24 Protein, Fc Tag
	CD4-H5257	Human	Human CD24 Protein, Mouse IgG2a Fc Tag
	CD4-H52H3	Human	Human CD24 Protein, His Tag
	CD4-H52H5	Human	Human CD24 Protein, His Tag (Nanoparticle)
	CD4-M52H7	Mouse	Mouse CD24 Protein, His Tag
	CD4-C8254	Cynomolgus	Biotinylated Cynomolgus CD24 Protein, Fc Tag, ultra sensitivity (primary amine labeling)
	CD4-C5257	Cynomolgus	Cynomolgus CD24 Protein, Fc Tag
	CD4-C52H5	Cynomolgus	Cynomolgus CD24 Protein, His Tag
Siglec-10	SI0-HP2E5	Human	PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay)^HOT
	SI0-H82E3	Human	Biotinylated Human Siglec-10 Protein, His,Avitag™
	SI0-H82F6	Human	Biotinylated Human Siglec-10 Protein, Fc,Avitag™ (MALS verified)
	SI0-H5253	Human	Human Siglec-10 Protein, Fc Tag
	SI0-H5254	Human	Human Siglec-10 Protein, Fc Tag
	SI0-H5255	Human	Human Siglec-10 Protein, Fc (L118A, G120A, E201A) Tag
	SI0-H525b	Human	Human Siglec-10 Protein, Mouse IgG2a Fc Tag, low endotoxin
	SI0-H52H9	Human	Human Siglec-10 Protein, His Tag (MALS verified)
	SI0-M52H7	Mouse	Mouse Siglec-10 Protein, His Tag
	SI0-C52H5	Cynomolgus	Cynomolgus Siglec-10 Protein, His Tag (MALS verified)

实验数据

CD24过表达细胞株与Siglec-10的结合验证

PE Labeled Siglec-10 Protein
: Fig.1 FACS analysis of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) at 1/10 dilution binding to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032).

Protocol

抗CD24抗体（SN3）抑制Siglec-10与CD24过表达细胞株的结合

Inhibition of Anti-CD24 Antibody (SN3)
: Fig.2 FACS analysis shows that the binding of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032) was inhibited by increasing concentration of neutralizing anti-CD24 antibody [SN3]. The IC50 of neutralizing anti-CD24 antibody [SN3] is 3.026 μg/ml.

Protocol

CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.

Authors: Barkal AA, et al.
Journal: Nature. 2019.
Download Full Article

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CD24 & Siglec-10蛋白 – 肿瘤免疫治疗热门靶点

CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.

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