【前沿进展】【Star Staining】CD19蛋白—多款荧光类型畅心选!

据相关文献显示，CD19仍然是当前细胞免疫疗法中最具研究热度的靶点之一，且已有多款CD19 CAR-T细胞疗法获批上市，关于靶向CD19的细胞疗法除了自体CAR-T疗法研究以外，还包括通用型CAR-T、多靶点CAR-T、CAR-NK疗法以及免疫检查点联合疗法等多种新型疗法模式，不断给肿瘤患者带来新希望。

为更好支持各种CD19 CAR新型细胞疗法开发，ACROBiosystems百普赛斯依托Star Staining定点标记技术平台开发了一系列独具特色的Star Staining荧光定点标记CD19蛋白产品，满足CD19细胞治疗药物在质控和临床样本分析中更高质量标准要求。

ACROBiosystems百普赛斯Star Staining新一代定点标记技术平台开发的高质量荧光标记蛋白，可最大程度保持蛋白天然构象，不影响蛋白活性，均一性好、批间一致性高。“星级”的研发实力，“星级”的技术平台及产品，Star Staining专为CAR-T细胞质控和临床样本分析检测而设计。>>>点击了解Star Staining相关信息

Star Staining荧光定点标记CD19蛋白产品，包括Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、FITC、APC、PE等多种荧光，对于单CAR、双CAR、多CAR等结构类型，您可畅心选择不同荧光，自由搭配，一步染色即出结果! >>>点击了解更多CD19产品信息

Star Staining荧光标记CD19蛋白系列产品，均经CD19类CAR细胞株FACS流式批检放行，可高特异、高灵敏检测CD19 CAR表达，具有高生物活性。

5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of AF488-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H3) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF488 signal was used to evaluate the binding activity.

Binding activity of FITC-Labeled Human CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human CD19 (Cat. No. CD9-HF2H3) protein has a much higher binding activity than that of the other competitor.

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为更好用于CD19 CAR细胞临床样本分析，Star Staining荧光标记CD19蛋白产品均经未转染CAR的PBMC细胞双染批检验证，无非特异背景问题，保证CAR检测结果的特异性和准确性。

5e5 of PBMCs were stained with AF488-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H3) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF488 signal was used to evaluate the non-specific binding activity to PBMCs.

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Star Staining荧光标记CD19蛋白产品不同批次蛋白活性可保持一致，具有高批间一致性，以确保长期用于CAR细胞质控和临床样本分析中检测结果的稳定性和可靠性。

Binding activity of different lots of FITC-Labeled Human CD19 (20-291), His Tag(Cat. No. CD9-HF2H3) against anti-CD19 CAR-293 cells was evaluated by flow cytometry. The result shows very high batch to batch consistency.

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FITC-Labeled Human CD19 (20-291), His Tag(Cat. No. CD9-HF2H3) is stable at 25℃ for 48 hours and Freeze-thaw 3 times.

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往期相关阅读

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