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Biotinylated Human ROR1 Protein, Avi Tag (Avitag™)

分子别名(Synonym)

ROR1,NTRKR1

表达区间及表达系统(Source)

Biotinylated Human ROR1, Avi Tag (RO1-H821y) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1).

Predicted N-terminus: Gln 30

蛋白结构(Molecular Characterization)

Online(Gln 30 - Glu 403) Q01973-1

This protein carries an Avi tag at the C-terminus.

The protein has a calculated MW of 45.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human ROR1, Avi Tag (Cat. No. RO1-H821y) SDS-PAGE gel

Biotinylated Human ROR1, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Please do not use together with anti human IgG Fc secondary antibody in order to avoid high background.

背景(Background)

Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.

 

前沿进展

货号/价格

RO1-H821y-25ug / ¥3500

RO1-H821y-200ug / ¥13800

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ROR1靶点信息
英文全称:Tyrosine-protein kinase transmembrane receptor ROR1
中文全称:蛋白酪氨酸激酶跨膜受体ROR1
种类:Mus musculus
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:
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