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Mouse BAFFR / TNFRSF13C Protein, Fc Tag (HPLC verified)

分子别名(Synonym)

BAFFR,TNFRSF13C,BAFF-R,BROMIX,CD268,CVID4,prolixin

表达区间及表达系统(Source)

Mouse BAFFR, Fc Tag (BAR-M5259) is expressed from human 293 cells (HEK293). It contains AA Ser 10 - Ala 71 (Accession # NP_082351.1).

Predicted N-terminus: Ser 10

蛋白结构(Molecular Characterization)

Online(Ser 10 - Ala 71) NP_082351.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 33.3 kDa. As a result of glycosylation, the protein migrates as 40-45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse BAFFR, Fc Tag (Cat. No. BAR-M5259) SDS-PAGE gel

Mouse BAFFR, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Mouse BAFFR, Fc Tag (Cat. No. BAR-M5259) HPLC images

The purity of Mouse BAFFR, Fc Tag (Cat. No. BAR-M5259) was greater than 95% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse BAFF, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BAF-M5257) at 2 μg/mL (100 μL/well) can bind Mouse BAFFR, Fc Tag (Cat. No. BAR-M5259) with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

背景(Background)

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

 

前沿进展

 
货号/价格

BAR-M5259-100ug / ¥3150

BAR-M5259-1mg (500ug X 2) / ¥22000

开发建议
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BAFFR靶点信息
英文全称:B-cell activating factor receptor
中文全称:B细胞活化因子受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床三期
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