膜杰作
Star Staining
分子别名（Synonym）
TMIGD2,IGPR1
表达区间及表达系统（Source）
Human CD28H, Fc Tag (CDH-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 150 (Accession # AAH15655.1).
Predicted N-terminus: Leu 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD28H, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD28H, Fc Tag (Cat. No. CDH-H5251) is more than 90% and the molecular weight of this protein is around 86-106 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H6) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human B7-H7, His Tag on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 16.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 13.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1,is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue,the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
