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Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag

分子别名(Synonym)

CSF3R,CD114,GCSFR

表达区间及表达系统(Source)

Biotinylated Human G-CSF R, Avitag,His Tag (GCR-H82E4) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751).

Predicted N-terminus: Glu 25

蛋白结构(Molecular Characterization)

Online(Glu 25 - Pro 621) NP_000751

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 69.2 kDa. The protein migrates as 72-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human G-CSF R, Avitag,His Tag (Cat. No. GCR-H82E4) SDS-PAGE gel

Biotinylated Human G-CSF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human G-CSF, Tag Free (Cat. No. GCF-H5214) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, Avitag,His Tag (Cat. No. GCR-H82E4) with a linear range of 0.156-1.25 μg/mL (QC tested).

Protocol

背景(Background)

The granulocyte colony-stimulating factor receptor (G-CSF-R) also known as CD114 (Cluster of Differentiation 114) is a protein that is encoded by the CSF3R gene. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). The G-CSF receptors belongs to a family of cytokine receptors known as the hematopoietin receptor family. Granulocyte colony-stimulating factor receptor has been shown to interact with Grb2, HCK and SHC1. The granulocyte colony-stimulating factor receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages.

 

前沿进展

 
货号/价格

GCR-H82E4-25ug / ¥3500

GCR-H82E4-200ug / ¥13800

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运输方式
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G-CSF R靶点信息
英文全称:Colony Stimulating Factor 3 Receptor
中文全称:巨噬细胞集落刺激因子3受体
种类:Homo sapiens
上市药物数量:95详情
临床药物数量:49详情
最高研发阶段:批准上市
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