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Biotinylated Human CD40 / TNFRSF5 Protein, Avitag™,His Tag (MALS verified)

分子别名(Synonym)

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统(Source)

Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (CD0-H82E8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).

Predicted N-terminus: Glu 21

蛋白结构(Molecular Characterization)

Online(Glu 21 - Arg 193) P25942-1

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No. CD0-H82E8) SDS-PAGE gel

Biotinylated Human CD40, Avitag™,His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No. CD0-H82E8) MALS images

The purity of Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No.CD0-H82E8) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS analysis shows that Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 3 μg/mL (Routinely tested).

Protocol

 

背景(Background)

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.

 

前沿进展

 
货号/价格

CD0-H82E8-25ug / ¥3500

CD0-H82E8-200ug / ¥13800

开发建议
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联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CD40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 5
中文全称:肿瘤坏死因子受体超家族成员5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:27详情
最高研发阶段:临床二期
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