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Biotinylated Human CD40 / TNFRSF5 Protein, Avitag™,His Tag (MALS verified)

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分子别名(Synonym)

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统(Source)

Biotinylated Human CD40 Protein, Avitag,His Tag (CD0-H82E8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).

Predicted N-terminus: Glu 21

Request for sequence

蛋白结构(Molecular Characterization)

CD40 Structure

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD40 SDS-PAGE

Biotinylated Human CD40 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

CD40 SEC-MALS

The purity of Biotinylated Human CD40 Protein, Avitag,His Tag (Cat. No. CD0-H82E8) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD40 ELISA

Immobilized Biotinylated Human CD40 Protein, Avitag,His Tag (Cat. No. CD0-H82E8) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5269) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

CD40 FACS

FACS analysis shows that Biotinylated Human CD40 Protein, Avitag,His Tag (Cat. No. CD0-H82E8) can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 3 μg/mL (Routinely tested).

Protocol

 

背景(Background)

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

文献引用(Citations)

 

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CD40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 5
中文全称:肿瘤坏死因子受体超家族成员5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:40详情
最高研发阶段:临床二期
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