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Biotinylated Human 4-1BB /TNFRSF9 Protein, Fc,Avitag™

分子别名(Synonym)

TNFRSF9,4-1BB,CD137,CDw137,ILA

表达区间及表达系统(Source)

Biotinylated Human 4-1BB, Fc,Avitag (41B-H82F7) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2).

Predicted N-terminus: Leu 24

蛋白结构(Molecular Characterization)

Online(Leu 24 - Gln 186) NP_001552.2

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 45.7 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F7) SDS-PAGE gel

Biotinylated Human 4-1BB, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 4‑1BB Ligand, Fc Tag (Cat. No. 41L-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F7) with a linear range of 2-19 ng/mL (QC tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

Flow Cytometry assay shows that Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F7) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.3 μg/mL (Routinely tested).

Protocol

 

背景(Background)

4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.

 

前沿进展

货号/价格

41B-H82F7-25ug / ¥3500

41B-H82F7-200ug / ¥13800

开发建议
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联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
4-1BB靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 9
中文全称:肿瘤坏死因子受体超家族成员9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:17详情
最高研发阶段:临床二期
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