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Human EGFRvIII Protein, His Tag (MALS verified)

分子别名(Synonym)

EGFRvIII

表达区间及表达系统(Source)

Human EGFRvIII, His Tag (EGI-H52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 378 (Accession # NP_001333870.1).

Predicted N-terminus: Leu 25

蛋白结构(Molecular Characterization)

Online(Leu 25 - Ser 378) NP_001333870.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 40.5 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) SDS-PAGE gel

Human EGFRvIII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) MALS images

The purity of Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) was more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-EGFRvIII Antibody , Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of anti-EGFRvIII CAR-293 cells were stained with 100 μL of 3 μg/mL of Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors; EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2–7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point

 

前沿进展

 
货号/价格

EGI-H52H4-50ug / ¥4100

EGI-H52H4-1mg (500ug X 2) / ¥22000

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EGFRvIII靶点信息
英文全称:Epidermal growth factor receptor variant III
中文全称:表皮生长因子受体变体III
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
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