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 >  Protein>TIGIT >TIT-R5258

Rat TIGIT Protein, Fc Tag

分子别名(Synonym)

TIGIT,VSIG9,VSTM3

表达区间及表达系统(Source)

Rat TIGIT, Fc Tag (TIT-R5258) is expressed from human 293 cells (HEK293). It contains AA Ala 17 - Ala 138 (Accession # D3ZTQ2).

Predicted N-terminus: Ala 17

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 17 - Ala 138) D3ZTQ2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 39.9 kDa. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 85-110 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rat TIGIT, Fc Tag (Cat. No. TIT-R5258) SDS-PAGE gel

Rat TIGIT, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 5 μg/mL (100 μL/well) can bind Rat TIGIT, Fc Tag (Cat. No. TIT-R5258) with a linear range of 0.039-5 μg/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Rat TIGIT, Fc Tag (Cat. No. TIT-R5258) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.59 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

 

 

前沿进展

 
 
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TIGIT靶点信息
英文全称:T cell immunoglobulin and ITIM domains
中文全称:T细胞免疫球蛋白ITIM结构域
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:39详情
最高研发阶段:临床三期
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