搜索
 >  其他蛋白>M-CSF R >CSR-C5252

Cynomolgus M-CSF R / CSF1R / CD115 Protein, Fc Tag

分子别名(Synonym)

CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR

表达区间及表达系统(Source)

Cynomolgus M-CSF R, Fc Tag (CSR-C5252) is expressed from human 293 cells (HEK293). It contains AA Ile 99 - Pro 596 (Accession # G7P8P1).

Predicted N-terminus: Ile 99

蛋白结构(Molecular Characterization)

Online(Ile 99 - Pro 596) G7P8P1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 81.9 kDa. As a result of glycosylation, the protein migrates as 110-125 kDa under reducing (R) condition, and 200- 240 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) SDS-PAGE gel

Cynomolgus M-CSF R, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/mL (100 μL/well) can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (Routinely tested).

Protocol

背景(Background)

Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

 

前沿进展

 
货号/价格

CSR-C5252-100ug / ¥2600

CSR-C5252-1mg / ¥16000

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

消息提示

请输入您的联系方式,再点击提交!

确定