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Zika virus NS1 Protein, His Tag

分子别名(Synonym)

NS1

表达区间及表达系统(Source)

Zika virus NS1, His Tag (NS1-Z5222) is expressed from human 293 cells (HEK293). It contains AA Val 796 - Ser 1148 (Accession # ALU33341).

Predicted N-terminus: Val 796

Request for sequence

蛋白结构(Molecular Characterization)

NS1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 42.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NS1 SDS-PAGE

Zika virus NS1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

背景(Background)

Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. The infection, known as Zika fever, often causes no or only mild symptoms, similar to a mild form of dengue fever. It is treated by rest. Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. As of 2016, the illness cannot be prevented by drugs or vaccines. As of February 2016, there is evidence that Zika fever in pregnant women is associated with abnormal brain development in their fetuses through mother-to-child transmission of the virus, which may result in miscarriage or microcephaly.

文献引用(Citations)

 

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NS1靶点信息
英文全称:Src homology phosphotyrosyl phosphatase 2
中文全称:蛋白酪氨酸磷酸酶-2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:16详情
最高研发阶段:临床二期
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