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Biotinylated Recombinant Protein L Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer)

分子别名(Synonym)

RPL,Protein L

表达区间及表达系统(Source)

Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (RPL-P814R) is expressed from E.coli cells. It is the biotinylated form of Recombinant Protein L, His Tag (RPL-P3141).

蛋白结构(Molecular Characterization)

Online()

This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 50-60 kDa on a SDS-PAGE gel under reducing (R) condition. This protein can bind to VL-Kappa.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 8-10.5 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (Cat. No. RPL-P814R) SDS-PAGE gel

Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human CTLA4 MAb at 5 μg/mL (100 μL/well)can bind Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (Cat. No. RPL-P814R) with a linear range of 0.1-1 ng/mL (QC tested).

点击申请Protocol

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-BCMA CAR Expression

CAR

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (Cat. No. RPL-P814R, 20 µg/mL), followed by FITC Streptavidin. FITC Streptavidin was used as negative control.

点击申请Protocol

 

背景(Background)

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

 

前沿进展

货号/价格

RPL-P814R-500ug / ¥800

RPL-P814R-2mg / ¥2200

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