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 >  Protein>TNF-alpha >TNA-M82E9

Biotinylated Mouse TNF-alpha Protein, His,Avitag™, active trimer (MALS verified)

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统(Source)

Biotinylated Mouse TNF-alpha Protein, His,Avitag (TNA-M82E9) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804 ).

Predicted N-terminus: Leu 80

Request for sequence

蛋白结构(Molecular Characterization)

TNF-alpha Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 20.2 kDa. The protein migrates as 20-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TNF-alpha SDS-PAGE

Biotinylated Mouse TNF-alpha Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

TNF-alpha SEC-MALS

The purity of Biotinylated Mouse TNF-alpha Protein, His,Avitag (Cat. No. TNA-M82E9) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

TNF-alpha ELISA

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse TNF-alpha Protein, His,Avitag (Cat. No. TNA-M82E9) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

文献引用(Citations)

 

前沿进展

 
 
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TNF-alpha靶点信息
英文全称:Tumor necrosis factor α
中文全称:肿瘤坏死因子α
种类:Homo sapiens
上市药物数量:61详情
临床药物数量:63详情
最高研发阶段:批准上市
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