Biotinylated Mouse TNF-alpha Protein, His,Avitag™ (active trimer) (MALS verified)

分子别名（Synonym）

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统（Source）

MABSol® Biotinylated Mouse TNF-alpha, His,Avitag (active trimer) (MALS verified) (TNA-M82E9) is expressed from human HEK293 cells. It contains AA Leu 80 - Leu 235 (Accession # P06804).

Predicted N-terminus: Leu 80

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 20.2 kDa. The protein migrates as 20-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

The biotin to protein ratio is 0.8-1 as determined by the HABA assay.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.