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Influenza A [A/Shanghai/2/2013(H7N9)] Hemagglutinin (HA) Protein, His Tag

分子别名(Synonym)

HA,Hemagglutinin

表达区间及表达系统(Source)

Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (HA9-V5227) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Gly 338, Leu 341 - Asp 523 (Accession # R4NN21-1).

Predicted N-terminus: Asp 19

蛋白结构(Molecular Characterization)

Online(Asp 19 - Gly 338, Leu 341 - Asp 523) R4NN21-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 56.7 kDa. The protein migrates as 63-75 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) SDS-PAGE gel

Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL (QC tested).

Protocol

背景(Background)

Influenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.

 

前沿进展

 
货号/价格

HA9-V5227-100ug / ¥2600

HA9-V5227-1mg / ¥15000

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