Glycoprotein B,gB,Envelope glycoprotein B
HCMV Glycoprotein B (gB), Fc Tag (CMB-V5255) Val 23 - Lys 700 (Accession # P13201-1) with furin cleavage site mutated from 'RTKR' to 'TTQT', was produced in human 293 cells (HEK293) at ACROBiosystems.
Predicted N-terminus: Val 23
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 104.0 kDa. The protein migrates as 140-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
HCMV Glycoprotein B (gB), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.