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 >  免疫检查点蛋白>Biotinylated Mouse PD-L1 / B7-H1, Fc Tag, Avi Tag (Avitag™)
货号/价格

PD1-M82F5-25ug / ¥3500

PD1-M82F5-200ug / ¥13800

联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
问题&评论

Biotinylated Mouse PD-L1 / B7-H1, Fc Tag, Avi Tag (Avitag™)

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

MABSol® Biotinylated Mouse PD-L1 / B7-H1, Fc Tag (PD1-M82F5) is expressed from human HEK293 cells. It contains AA Phe 19 - Thr 238 (Accession # NP_068693).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

PD-L1(Phe 19 - Thr 238) NP_068693

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 53.2 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

生物素标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

生物素-蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Mouse PD-L1, Fc Tag (Cat. No. PD1-M82F5) SDS-PAGE gel

Biotinylated Mouse PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)

Biotinylated Mouse PD-L1, Fc Tag (Cat. No. PD1-M82F5) ELISA bioactivity

Immobilized Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) at 10 μg/mL (100 µl/well),can bind Biotinylated Mouse PD-L1, Fc Tag (Cat. No. PD1-M82F5) with a linear range of 0.15-2.5 μg/mL (QC tested).

点击申请Protocol

 

背景(Background)

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

参考文献(References)

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

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