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Rat FcRn/FCGRT & B2M Heterodimer Protein

分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Rat FcRn / FCGRT & B2M Heterodimer Protein, Ala 23 - Ser 298 (FCGRT, NP_203502.1) and & Ile 21 - Met 119 (B2M, NP_036644), was produced in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)

蛋白结构(Molecular Characterization)

Online(Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_203502.1 (FCGRT) & NP_036644 (B2M)

Rat FcRn / FCGRT & B2M Heterodimer Protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 23 (FCGRT) & Ile 21 (B2M). The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14.5 kDa (B2M) respectively due to different glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rat FcRn, His Tag, Strep Tag (Cat. No. FCM-R5287) SDS-PAGE gel

Rat FcRn, His Tag, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

文献引用(Citations)

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

前沿进展

货号/价格

FCM-R5287-50ug / ¥3500

FCM-R5287-1mg / ¥24000

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