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Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)

分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) (FCM-P5280) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M) (Accession # Q6XAV9-1(FCGRT) & Q07717-1(B2M)).

Predicted N-terminus: Asp 16 (FCGRT) & Val 21 (B2M)

蛋白结构(Molecular Characterization)

Online(Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M)) Q6XAV9-1 (FCGRT) & Q07717-1 (B2M)

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 12.8 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 32-35 kDa (FCGRT) and 14 kDa (B2M) respectively.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-P5280) SDS-PAGE gel

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

活性(Bioactivity)-SPR

Biotinylated Human SPR

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-P5280) captured on CM5 Chip via anti-His antibody can bind Herceptin® with an affinity constant of 13.8 nM as determined in SPR assay (Biacore 8K) (QC tested).

Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

前沿进展

 
货号/价格

FCM-P5280-50ug / ¥3150

FCM-P5280-1mg (250ug X 4) / ¥24000

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