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Biotinylated Human Transferrin R / CD71 Protein, His Tag, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90

表达区间及表达系统(Source)

Biotinylated Human Transferrin R, His Tag, primary amine labeling is expressed from human HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R protein, His Tag (Cat. No. CD1-H5243).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Online(Cys 89 - Phe 760) AAH01188.1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 76.0 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) SDS-PAGE gel

Biotinylated Human Transferrin R, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Transferrin, His Tag (Cat. No. TRN-H4229) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) with a linear range of 5-156 ng/mL (QC tested).

Protocol

背景(Background)

CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

 

前沿进展

 
货号/价格

TFR-H8243-25ug / ¥2900

TFR-H8243-200ug / ¥12500

开发建议
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021-26136428(上海)
运输方式
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Transferrin R靶点信息
英文全称:Transferrin receptor protein 1
中文全称:转铁蛋白受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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