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 >  Protein>VEGF-C >VEC-H4225

Human VEGF-C / Flt4-L Protein, His Tag (MALS verified)

分子别名(Synonym)

VEGFC,Flt4-L,VRP

表达区间及表达系统(Source)

Human VEGF-C, His Tag (VEC-H4225) is expressed from human 293 cells (HEK293). It contains AA Thr 103 - Arg 227 (Accession # AAH35212.1 ).

Predicted N-terminus: Thr 103

Request for sequence

蛋白结构(Molecular Characterization)

VEGF-C Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 14.9 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

VEGF-C SDS-PAGE

Human VEGF-C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

VEGF-C SEC-MALS

The purity of Human VEGF-C, His Tag (Cat. No. VEC-H4225) is more than 95% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

VEGF-C ELISA

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-C, His Tag (Cat. No. VEC-H4225) with a linear range of 2-20 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

VEGF-C SPR

Human VEGF-C, His Tag (Cat. No. VEC-H4225) captured on CM5 Chip via anti-His antibody can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with an affinity constant of 41.1 pM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP, it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.

文献引用(Citations)

 

前沿进展

 
 
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VEGF-C靶点信息
英文全称:Vascular endothelial growth factor C
中文全称:血管内皮生长因子C
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床三期
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