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Human VEGF R2 / KDR Protein, Strep Tag

分子别名(Synonym)

KDR,CD309,FLK1,VEGFR,VEGFR2

表达区间及表达系统(Source)

Human VEGF R2, Strep Tag (KDR-H5280) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823).

Predicted N-terminus: Ala 20

蛋白结构(Molecular Characterization)

Online(Ala 20 - Glu 764) AAI31823

This protein carries a twin strep tag at the C-terminus.

The protein has a calculated MW of 86.3 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human VEGF R2, Strep Tag (Cat. No. KDR-H5280) SDS-PAGE gel

Human VEGF R2, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human VEGF121, Tag Free (Cat. No. VE1-H4213) at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag (Cat. No. KDR-H5280) with a linear range of 0.15-2.5 μg/mL (QC tested).

Protocol

背景(Background)

Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.

 

前沿进展

 
货号/价格

KDR-H5280-25ug / ¥2600

KDR-H5280-250ug / ¥8800

KDR-H5280-1mg (250ug X 4) / ¥23000

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