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Human uPAR / PLAUR Protein, His Tag

分子别名(Synonym)

uPAR,PLAUR,CD87,MO3

表达区间及表达系统(Source)

Human uPAR, His Tag (UPR-H5226) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # AAH02788).

Predicted N-terminus: Leu 23

蛋白结构(Molecular Characterization)

Online(Leu 23 - Arg 303) AAH02788

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human uPAR, His Tag (Cat. No. UPR-H5226) SDS-PAGE gel

Human uPAR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Measured by its inhibitory ability in a competitive ELISA.
Serial dilutions of Human uPAR, His Tag (Cat. No. UPR-H5226) were added into Human PLAU, His Tag (Cat. No. PLU-H5229) : Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) binding reactions. The half maximal inhibitory concentration (IC50) is 2.96 μg/mL (QC tested).

Protocol

背景(Background)

Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.

 

前沿进展

 
货号/价格

UPR-H5226-200ug / ¥2500

UPR-H5226-1mg (500ug X 2) / ¥9800

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